PRIMARY FUNCTION:

Staff Scientist II will utilize their Molecular Biology experience and knowledge to work with a cross-functional team of cell and molecular biologists within the Gene Editing Institute. The successful candidate is expected to carry out the assigned projects mostly independently. Primary responsibilities include but are not limited to developing, optimizing, executing and analyzing in-silico, in-vitro and in-vivo CRISPR off-target assays for gene therapy products.

PRINCIPAL DUTIES AND RESPONSIBILITIES:

Design and optimize PCR reactions

Restriction digestion, conventional and seamless cloning

Perform total RNA and genomic DNA purification from mammalian cells

Carry out Sanger and next generation sequencing (NGS)

Perform transfection and/or transduction of mammalian cells utilizing nucleofection and/or AAV

Interpretation of Sanger and NGS data

Close communication with all involved team members and supervisors

Maintenance of laboratory equipment

Maintenance of a highly organized, technical and detailed laboratory notebook containing protocols used, results derived from each experiment or measurement

Preparation and dissemination of technical reports

Present research findings, including preparation of research reports and abstracts, dissemination at national and international scientific conferences and publication of manuscripts by peer-reviewed scientific journals.

Maintain familiarity with current scientific literature and techniques in the field of CRISPR based gene therapy

Perform assigned work safely, adhering to established departmental safety rules and practices; report to Group leader, in a timely manner, any unsafe activities, conditions, hazards, or safety violations that may cause injury to oneself, other employees, patients and visitors.

Performs other related duties as required/assigned.

SCOPE, PURPOSE, AND FREQUENCY OF CONTACTS:

Scientific opinions and discussion to collaborators.

DIRECTION/SUPERVISION OF OTHERS:

Limited supervision of laboratory assistants

DIRECTION/SUPERVISION RECEIVED:

Group leader / Director of Gene Editing Institute

EDUCATION AND EXPERIENCE REQUIREMENTS:

Bachelor's degree in molecular biology, biochemistry, biological sciences or related field with 3 - 7 years of work experience or MS with 0 - 4 years of work experience

Extensive experience with wide variety of molecular biology techniques

Experience with Sanger and Next-generation sequencing and analysis is a plus

Experience in fast-paced biotechnology industry is preferred

Experience in cell and molecular biology assay development

Experience with managing multiple projects simultaneously

KNOWLEDGE, SKILL, AND ABILITY REQUIREMENTS:

Strong knowledge and skill base of molecular biology methods

Well-versed in culturing mammalian cells is a plus

Strong troubleshooting skills

Adaptable to incorporating new methods to improve various aspects of workflows

Ability to effectively work independently and as part of a team

Skilled in effective communication and reporting with team members and supervisors

Knowledge in CRISPR genome engineering

Experienced in in vitro assays using immortalized cancer cells

Experienced in cancer biology/genetics

SPECIAL REQUIREMENTS:

n/a

PHYSICAL DEMANDS:

Frequent sitting, standing and walking with or without reasonable accommodation for physical disabilities

WORKING CONDITIONS:

Office, clinical and laboratory settings